Isolation and In Vitro Characterization of Epidermal Stem Cells
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Colony-forming assays represent prospective methods, where cells isolated from enzymatically dissociated tissues or from tissue cultures are assessed for their proliferative capacity in vitro. Complex tissues such as the epithelial component of the skin (the epidermis) are characterized by a substantial cellular heterogeneity. Analysis of bulk populations of cells by colony-forming assays can consequently be convoluted by a number of factors that are not controlled for in population wide studies. It is therefore advantageous to refine in vitro growth assays by sub-fractionation of cells using flow cytometry. Using markers that define the spatial origin of epidermal cells, it is possible to interrogate the specific characteristics of subpopulations of cells based on their in vivo credentials. Here, we describe how to isolate, culture, and characterize keratinocytes from murine back and tail skin sorted by surface antigens associated with adult stem cell characteristics.
Original language | English |
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Title of host publication | Adult Stem Cells : Methods in Molecular Biology |
Editors | Di Nardo Paolo, Dhingra Sinjiv, Singla Dinender K. |
Number of pages | 17 |
Volume | 1553 |
Publisher | Humana Press |
Publication date | 2017 |
Pages | 67-83 |
ISBN (Print) | 978-1-4939-6754-4 |
ISBN (Electronic) | 978-1-4939-6756-8 |
DOIs | |
Publication status | Published - 2017 |
Series | Methods in molecular biology (Clifton, N.J.) |
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ISSN | 1064-3745 |
ID: 184638850