Isolation and In Vitro Characterization of Epidermal Stem Cells

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Isolation and In Vitro Characterization of Epidermal Stem Cells. / Moestrup, Kasper S; Andersen, Marianne Stemann; Jensen, Kim Bak.

Adult Stem Cells: Methods in Molecular Biology. ed. / Di Nardo Paolo; Dhingra Sinjiv; Singla Dinender K. Vol. 1553 Humana Press, 2017. p. 67-83 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Moestrup, KS, Andersen, MS & Jensen, KB 2017, Isolation and In Vitro Characterization of Epidermal Stem Cells. in DN Paolo, D Sinjiv & S Dinender K. (eds), Adult Stem Cells: Methods in Molecular Biology. vol. 1553, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 67-83. https://doi.org/10.1007/978-1-4939-6756-8_6

APA

Moestrup, K. S., Andersen, M. S., & Jensen, K. B. (2017). Isolation and In Vitro Characterization of Epidermal Stem Cells. In D. N. Paolo, D. Sinjiv, & S. Dinender K. (Eds.), Adult Stem Cells: Methods in Molecular Biology (Vol. 1553, pp. 67-83). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-4939-6756-8_6

Vancouver

Moestrup KS, Andersen MS, Jensen KB. Isolation and In Vitro Characterization of Epidermal Stem Cells. In Paolo DN, Sinjiv D, Dinender K. S, editors, Adult Stem Cells: Methods in Molecular Biology. Vol. 1553. Humana Press. 2017. p. 67-83. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-4939-6756-8_6

Author

Moestrup, Kasper S ; Andersen, Marianne Stemann ; Jensen, Kim Bak. / Isolation and In Vitro Characterization of Epidermal Stem Cells. Adult Stem Cells: Methods in Molecular Biology. editor / Di Nardo Paolo ; Dhingra Sinjiv ; Singla Dinender K. Vol. 1553 Humana Press, 2017. pp. 67-83 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{3c1fbd4d4831416dadbfc06fc957c07c,
title = "Isolation and In Vitro Characterization of Epidermal Stem Cells",
abstract = "Colony-forming assays represent prospective methods, where cells isolated from enzymatically dissociated tissues or from tissue cultures are assessed for their proliferative capacity in vitro. Complex tissues such as the epithelial component of the skin (the epidermis) are characterized by a substantial cellular heterogeneity. Analysis of bulk populations of cells by colony-forming assays can consequently be convoluted by a number of factors that are not controlled for in population wide studies. It is therefore advantageous to refine in vitro growth assays by sub-fractionation of cells using flow cytometry. Using markers that define the spatial origin of epidermal cells, it is possible to interrogate the specific characteristics of subpopulations of cells based on their in vivo credentials. Here, we describe how to isolate, culture, and characterize keratinocytes from murine back and tail skin sorted by surface antigens associated with adult stem cell characteristics.",
author = "Moestrup, {Kasper S} and Andersen, {Marianne Stemann} and Jensen, {Kim Bak}",
year = "2017",
doi = "10.1007/978-1-4939-6756-8_6",
language = "English",
isbn = "978-1-4939-6754-4",
volume = "1553",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "67--83",
editor = "Paolo, {Di Nardo} and Dhingra Sinjiv and {Dinender K.}, Singla",
booktitle = "Adult Stem Cells",
address = "United States",

}

RIS

TY - CHAP

T1 - Isolation and In Vitro Characterization of Epidermal Stem Cells

AU - Moestrup, Kasper S

AU - Andersen, Marianne Stemann

AU - Jensen, Kim Bak

PY - 2017

Y1 - 2017

N2 - Colony-forming assays represent prospective methods, where cells isolated from enzymatically dissociated tissues or from tissue cultures are assessed for their proliferative capacity in vitro. Complex tissues such as the epithelial component of the skin (the epidermis) are characterized by a substantial cellular heterogeneity. Analysis of bulk populations of cells by colony-forming assays can consequently be convoluted by a number of factors that are not controlled for in population wide studies. It is therefore advantageous to refine in vitro growth assays by sub-fractionation of cells using flow cytometry. Using markers that define the spatial origin of epidermal cells, it is possible to interrogate the specific characteristics of subpopulations of cells based on their in vivo credentials. Here, we describe how to isolate, culture, and characterize keratinocytes from murine back and tail skin sorted by surface antigens associated with adult stem cell characteristics.

AB - Colony-forming assays represent prospective methods, where cells isolated from enzymatically dissociated tissues or from tissue cultures are assessed for their proliferative capacity in vitro. Complex tissues such as the epithelial component of the skin (the epidermis) are characterized by a substantial cellular heterogeneity. Analysis of bulk populations of cells by colony-forming assays can consequently be convoluted by a number of factors that are not controlled for in population wide studies. It is therefore advantageous to refine in vitro growth assays by sub-fractionation of cells using flow cytometry. Using markers that define the spatial origin of epidermal cells, it is possible to interrogate the specific characteristics of subpopulations of cells based on their in vivo credentials. Here, we describe how to isolate, culture, and characterize keratinocytes from murine back and tail skin sorted by surface antigens associated with adult stem cell characteristics.

U2 - 10.1007/978-1-4939-6756-8_6

DO - 10.1007/978-1-4939-6756-8_6

M3 - Book chapter

C2 - 28229408

SN - 978-1-4939-6754-4

VL - 1553

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 67

EP - 83

BT - Adult Stem Cells

A2 - Paolo, Di Nardo

A2 - Sinjiv, Dhingra

A2 - Dinender K., Singla

PB - Humana Press

ER -

ID: 184638850