Identification of keratinocyte-specific markers using phage display and mass spectrometry
Research output: Contribution to journal › Journal article › Research › peer-review
Specific molecular markers for various normal and pathogenic cell states and cell types provide knowledge of basic biological systems and have a direct application in targeted therapy. We describe a proteomic method based on the combination of new and improved phage display antibody technologies and mass spectrometry that allows identification of cell type-specific protein markers. The most important features of the method are (i) reduction of experimental noise originating from background binding of phage particles and (ii) isolation of affinity binders after a single round of selection, which assures a high diversity of binders. The method demonstrates, for the first time, the ability to detect, identify, and analyze both secreted and membrane-associated extracellular proteins as well as a variety of different cellular structures including proteins and carbohydrates. The optimized phage display method was applied to analysis of human skin keratinocytes resulting in the isolation of a panel of antibodies. Fourteen of these antibodies were further characterized, half of which predominantly recognized keratinocytes in a screen of a range of different cell types. Three cognate keratinocyte antigens were subsequently identified by mass spectrometry as laminin-5, plectin, and fibronectin. The combination of phage display technology with mass spectrometry methods for protein identification is a general and promising approach for proteomic analysis of cell surface complexity.
Original language | English |
---|---|
Journal | Molecular and Cellular Proteomics |
Volume | 2 |
Issue number | 2 |
Pages (from-to) | 61-9 |
Number of pages | 9 |
ISSN | 1535-9476 |
DOIs | |
Publication status | Published - Feb 2003 |
Externally published | Yes |
- Antibodies, Monoclonal, Breast/cytology, Cell Line, Cell Line, Tumor, Cells, Cultured, Coliphages/genetics, Epitopes/analysis, Escherichia coli/genetics, Genetic Markers, HeLa Cells, Humans, Intermediate Filament Proteins/genetics, Keratinocytes/physiology, Laminin/genetics, Mass Spectrometry, Peptide Library, Plectin, Sensitivity and Specificity, Skin Physiological Phenomena, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Research areas
ID: 200572520