A BAC transgenic Hes1-EGFP reporter reveals novel expression domains in mouse embryos

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A BAC transgenic Hes1-EGFP reporter reveals novel expression domains in mouse embryos. / Klinck, Rasmus; Füchtbauer, Ernst-Martin; Ahnfelt-Rønne, Jonas; Serup, Palle; Jensen, Jan Nygaard; Jørgensen, Mette Christine.

In: Gene Expression Patterns, Vol. 11, No. 7, 10.2011, p. 415-26.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Klinck, R, Füchtbauer, E-M, Ahnfelt-Rønne, J, Serup, P, Jensen, JN & Jørgensen, MC 2011, 'A BAC transgenic Hes1-EGFP reporter reveals novel expression domains in mouse embryos', Gene Expression Patterns, vol. 11, no. 7, pp. 415-26. https://doi.org/10.1016/j.gep.2011.06.004

APA

Klinck, R., Füchtbauer, E-M., Ahnfelt-Rønne, J., Serup, P., Jensen, J. N., & Jørgensen, M. C. (2011). A BAC transgenic Hes1-EGFP reporter reveals novel expression domains in mouse embryos. Gene Expression Patterns, 11(7), 415-26. https://doi.org/10.1016/j.gep.2011.06.004

Vancouver

Klinck R, Füchtbauer E-M, Ahnfelt-Rønne J, Serup P, Jensen JN, Jørgensen MC. A BAC transgenic Hes1-EGFP reporter reveals novel expression domains in mouse embryos. Gene Expression Patterns. 2011 Oct;11(7):415-26. https://doi.org/10.1016/j.gep.2011.06.004

Author

Klinck, Rasmus ; Füchtbauer, Ernst-Martin ; Ahnfelt-Rønne, Jonas ; Serup, Palle ; Jensen, Jan Nygaard ; Jørgensen, Mette Christine. / A BAC transgenic Hes1-EGFP reporter reveals novel expression domains in mouse embryos. In: Gene Expression Patterns. 2011 ; Vol. 11, No. 7. pp. 415-26.

Bibtex

@article{70575ff9ae3b40cd864df7f1499c8b5d,
title = "A BAC transgenic Hes1-EGFP reporter reveals novel expression domains in mouse embryos",
abstract = "Expression of the basic helix-loop-helix factor Hairy and Enhancer of Split-1 (Hes1) is required for normal development of a number of tissues during embryonic development. Depending on context, Hes1 may act as a Notch signalling effector which promotes the undifferentiated and proliferative state of progenitor cells, but increasing evidence also points to Notch independent regulation of Hes1 expression. Here we use high resolution confocal scanning of EGFP in a novel BAC transgenic mouse reporter line, Tg(Hes1-EGFP)(1Hri), to analyse Hes1 expression from embryonic day 7.0 (e7.0). Our data recapitulates some previous observations on Hes1 expression and suggests new, hitherto unrecognised expression domains including expression in the definitive endoderm at early somite stages before gut tube closure and thus preceding organogenesis. This mouse line will be a valuable tool for studies addressing the role of Hes1 in a number of different research areas including organ specification, development and regeneration.",
keywords = "Animals, Basic Helix-Loop-Helix Transcription Factors, Chromosomes, Artificial, Bacterial, Endoderm, Gene Expression Regulation, Developmental, Green Fluorescent Proteins, Homeodomain Proteins, Mice, Mice, Transgenic, Organogenesis, Somites",
author = "Rasmus Klinck and Ernst-Martin F{\"u}chtbauer and Jonas Ahnfelt-R{\o}nne and Palle Serup and Jensen, {Jan Nygaard} and J{\o}rgensen, {Mette Christine}",
note = "Copyright {\textcopyright} 2011 Elsevier B.V. All rights reserved.",
year = "2011",
month = oct,
doi = "10.1016/j.gep.2011.06.004",
language = "English",
volume = "11",
pages = "415--26",
journal = "Gene Expression Patterns",
issn = "1567-133X",
publisher = "Elsevier",
number = "7",

}

RIS

TY - JOUR

T1 - A BAC transgenic Hes1-EGFP reporter reveals novel expression domains in mouse embryos

AU - Klinck, Rasmus

AU - Füchtbauer, Ernst-Martin

AU - Ahnfelt-Rønne, Jonas

AU - Serup, Palle

AU - Jensen, Jan Nygaard

AU - Jørgensen, Mette Christine

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2011/10

Y1 - 2011/10

N2 - Expression of the basic helix-loop-helix factor Hairy and Enhancer of Split-1 (Hes1) is required for normal development of a number of tissues during embryonic development. Depending on context, Hes1 may act as a Notch signalling effector which promotes the undifferentiated and proliferative state of progenitor cells, but increasing evidence also points to Notch independent regulation of Hes1 expression. Here we use high resolution confocal scanning of EGFP in a novel BAC transgenic mouse reporter line, Tg(Hes1-EGFP)(1Hri), to analyse Hes1 expression from embryonic day 7.0 (e7.0). Our data recapitulates some previous observations on Hes1 expression and suggests new, hitherto unrecognised expression domains including expression in the definitive endoderm at early somite stages before gut tube closure and thus preceding organogenesis. This mouse line will be a valuable tool for studies addressing the role of Hes1 in a number of different research areas including organ specification, development and regeneration.

AB - Expression of the basic helix-loop-helix factor Hairy and Enhancer of Split-1 (Hes1) is required for normal development of a number of tissues during embryonic development. Depending on context, Hes1 may act as a Notch signalling effector which promotes the undifferentiated and proliferative state of progenitor cells, but increasing evidence also points to Notch independent regulation of Hes1 expression. Here we use high resolution confocal scanning of EGFP in a novel BAC transgenic mouse reporter line, Tg(Hes1-EGFP)(1Hri), to analyse Hes1 expression from embryonic day 7.0 (e7.0). Our data recapitulates some previous observations on Hes1 expression and suggests new, hitherto unrecognised expression domains including expression in the definitive endoderm at early somite stages before gut tube closure and thus preceding organogenesis. This mouse line will be a valuable tool for studies addressing the role of Hes1 in a number of different research areas including organ specification, development and regeneration.

KW - Animals

KW - Basic Helix-Loop-Helix Transcription Factors

KW - Chromosomes, Artificial, Bacterial

KW - Endoderm

KW - Gene Expression Regulation, Developmental

KW - Green Fluorescent Proteins

KW - Homeodomain Proteins

KW - Mice

KW - Mice, Transgenic

KW - Organogenesis

KW - Somites

U2 - 10.1016/j.gep.2011.06.004

DO - 10.1016/j.gep.2011.06.004

M3 - Journal article

C2 - 21745596

VL - 11

SP - 415

EP - 426

JO - Gene Expression Patterns

JF - Gene Expression Patterns

SN - 1567-133X

IS - 7

ER -

ID: 46484188