Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors

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Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors. / Sachdeva, Rohit; Jönsson, Marie E.; Nelander, Jenny; Kirkeby, Agnete; Guibentif, Carolina; Gentner, Bernhard; Naldini, Luigi; Björklund, Anders; Parmar, Malin; Jakobsson, Johan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 25, 22.06.2010, p. 11602-11607.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sachdeva, R, Jönsson, ME, Nelander, J, Kirkeby, A, Guibentif, C, Gentner, B, Naldini, L, Björklund, A, Parmar, M & Jakobsson, J 2010, 'Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 25, pp. 11602-11607. https://doi.org/10.1073/pnas.1006568107

APA

Sachdeva, R., Jönsson, M. E., Nelander, J., Kirkeby, A., Guibentif, C., Gentner, B., Naldini, L., Björklund, A., Parmar, M., & Jakobsson, J. (2010). Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors. Proceedings of the National Academy of Sciences of the United States of America, 107(25), 11602-11607. https://doi.org/10.1073/pnas.1006568107

Vancouver

Sachdeva R, Jönsson ME, Nelander J, Kirkeby A, Guibentif C, Gentner B et al. Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors. Proceedings of the National Academy of Sciences of the United States of America. 2010 Jun 22;107(25):11602-11607. https://doi.org/10.1073/pnas.1006568107

Author

Sachdeva, Rohit ; Jönsson, Marie E. ; Nelander, Jenny ; Kirkeby, Agnete ; Guibentif, Carolina ; Gentner, Bernhard ; Naldini, Luigi ; Björklund, Anders ; Parmar, Malin ; Jakobsson, Johan. / Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 25. pp. 11602-11607.

Bibtex

@article{1a32093a1b644ddbb7cee15120092dda,
title = "Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors",
abstract = "In this study, we have used a microRNA-regulated lentiviral reporter system to visualize and segregate differentiating neuronal cells in pluripotent cultures. Efficient suppression of transgene expression, specifically in undifferentiated pluripotent cells, was achieved by using a lentiviral vector expressing a fluorescent reporter gene regulated by microRNA-292. Using this strategy, it was possible to track progeny from murine ES, human ES cells, and induced pluripotent stem cells as they differentiated toward the neural lineage. In addition, this strategy was successfully used to FACS purify neuronal progenitors for molecular analysis and transplantation. FACS enrichment reduced tumor formation and increased survival of ES cell-derived neuronal progenitors after transplantation. The properties and versatility of the microRNA-regulated vectors allows broad use of these vectors in stem cell applications.",
keywords = "ES, Induced pluripotent stem cells, Stem cells, Transplantation",
author = "Rohit Sachdeva and J{\"o}nsson, {Marie E.} and Jenny Nelander and Agnete Kirkeby and Carolina Guibentif and Bernhard Gentner and Luigi Naldini and Anders Bj{\"o}rklund and Malin Parmar and Johan Jakobsson",
year = "2010",
month = jun,
day = "22",
doi = "10.1073/pnas.1006568107",
language = "English",
volume = "107",
pages = "11602--11607",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "25",

}

RIS

TY - JOUR

T1 - Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors

AU - Sachdeva, Rohit

AU - Jönsson, Marie E.

AU - Nelander, Jenny

AU - Kirkeby, Agnete

AU - Guibentif, Carolina

AU - Gentner, Bernhard

AU - Naldini, Luigi

AU - Björklund, Anders

AU - Parmar, Malin

AU - Jakobsson, Johan

PY - 2010/6/22

Y1 - 2010/6/22

N2 - In this study, we have used a microRNA-regulated lentiviral reporter system to visualize and segregate differentiating neuronal cells in pluripotent cultures. Efficient suppression of transgene expression, specifically in undifferentiated pluripotent cells, was achieved by using a lentiviral vector expressing a fluorescent reporter gene regulated by microRNA-292. Using this strategy, it was possible to track progeny from murine ES, human ES cells, and induced pluripotent stem cells as they differentiated toward the neural lineage. In addition, this strategy was successfully used to FACS purify neuronal progenitors for molecular analysis and transplantation. FACS enrichment reduced tumor formation and increased survival of ES cell-derived neuronal progenitors after transplantation. The properties and versatility of the microRNA-regulated vectors allows broad use of these vectors in stem cell applications.

AB - In this study, we have used a microRNA-regulated lentiviral reporter system to visualize and segregate differentiating neuronal cells in pluripotent cultures. Efficient suppression of transgene expression, specifically in undifferentiated pluripotent cells, was achieved by using a lentiviral vector expressing a fluorescent reporter gene regulated by microRNA-292. Using this strategy, it was possible to track progeny from murine ES, human ES cells, and induced pluripotent stem cells as they differentiated toward the neural lineage. In addition, this strategy was successfully used to FACS purify neuronal progenitors for molecular analysis and transplantation. FACS enrichment reduced tumor formation and increased survival of ES cell-derived neuronal progenitors after transplantation. The properties and versatility of the microRNA-regulated vectors allows broad use of these vectors in stem cell applications.

KW - ES

KW - Induced pluripotent stem cells

KW - Stem cells

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=77954923288&partnerID=8YFLogxK

U2 - 10.1073/pnas.1006568107

DO - 10.1073/pnas.1006568107

M3 - Journal article

C2 - 20534548

AN - SCOPUS:77954923288

VL - 107

SP - 11602

EP - 11607

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 25

ER -

ID: 228506424