Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis
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Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis. / Driskell, Ryan R; Giangreco, Adam; Jensen, Kim B; Mulder, Klaas W; Watt, Fiona M.
In: Development (Cambridge, England), Vol. 136, No. 16, 08.2009, p. 2815-23.Research output: Contribution to journal › Journal article › Research
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TY - JOUR
T1 - Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis
AU - Driskell, Ryan R
AU - Giangreco, Adam
AU - Jensen, Kim B
AU - Mulder, Klaas W
AU - Watt, Fiona M
PY - 2009/8
Y1 - 2009/8
N2 - The dermal papilla comprises the specialised mesenchymal cells at the base of the hair follicle. Communication between dermal papilla cells and the overlying epithelium is essential for differentiation of the hair follicle lineages. We report that Sox2 is expressed in all dermal papillae at E16.5, but from E18.5 onwards expression is confined to a subset of dermal papillae. In postnatal skin, Sox2 is only expressed in the dermal papillae of guard/awl/auchene follicles, whereas CD133 is expressed both in guard/awl/auchene and in zigzag dermal papillae. Using transgenic mice that express GFP under the control of the Sox2 promoter, we isolated Sox2(+) (GFP(+)) CD133(+) cells and compared them with Sox2(-) (GFP(-)) CD133(+) dermal papilla cells. In addition to the 'core' dermal papilla gene signature, each subpopulation expressed distinct sets of genes. GFP(+) CD133(+) cells had upregulated Wnt, FGF and BMP pathways and expressed neural crest markers. In GFP(-) CD133(+) cells, the hedgehog, IGF, Notch and integrin pathways were prominent. In skin reconstitution assays, hair follicles failed to form when dermis was depleted of both GFP(+) CD133(+) and GFP(-) CD133(+) cells. In the absence of GFP(+) CD133(+) cells, awl/auchene hairs failed to form and only zigzag hairs were found. We have thus demonstrated a previously unrecognised heterogeneity in dermal papilla cells and shown that Sox2-positive cells specify particular hair follicle types.
AB - The dermal papilla comprises the specialised mesenchymal cells at the base of the hair follicle. Communication between dermal papilla cells and the overlying epithelium is essential for differentiation of the hair follicle lineages. We report that Sox2 is expressed in all dermal papillae at E16.5, but from E18.5 onwards expression is confined to a subset of dermal papillae. In postnatal skin, Sox2 is only expressed in the dermal papillae of guard/awl/auchene follicles, whereas CD133 is expressed both in guard/awl/auchene and in zigzag dermal papillae. Using transgenic mice that express GFP under the control of the Sox2 promoter, we isolated Sox2(+) (GFP(+)) CD133(+) cells and compared them with Sox2(-) (GFP(-)) CD133(+) dermal papilla cells. In addition to the 'core' dermal papilla gene signature, each subpopulation expressed distinct sets of genes. GFP(+) CD133(+) cells had upregulated Wnt, FGF and BMP pathways and expressed neural crest markers. In GFP(-) CD133(+) cells, the hedgehog, IGF, Notch and integrin pathways were prominent. In skin reconstitution assays, hair follicles failed to form when dermis was depleted of both GFP(+) CD133(+) and GFP(-) CD133(+) cells. In the absence of GFP(+) CD133(+) cells, awl/auchene hairs failed to form and only zigzag hairs were found. We have thus demonstrated a previously unrecognised heterogeneity in dermal papilla cells and shown that Sox2-positive cells specify particular hair follicle types.
KW - Animals
KW - Antigens, CD
KW - Dermis
KW - Embryo, Mammalian
KW - Epidermis
KW - Gene Expression Profiling
KW - Gene Regulatory Networks
KW - Glycoproteins
KW - Hair Follicle
KW - Mice
KW - Mice, Inbred BALB C
KW - Mice, Nude
KW - Mice, Transgenic
KW - Oligonucleotide Array Sequence Analysis
KW - Peptides
KW - Recombinant Fusion Proteins
KW - SOXB1 Transcription Factors
KW - Signal Transduction
U2 - 10.1242/dev.038620
DO - 10.1242/dev.038620
M3 - Journal article
C2 - 19605494
VL - 136
SP - 2815
EP - 2823
JO - Development
JF - Development
SN - 0950-1991
IS - 16
ER -
ID: 94414506