Ribosomal RNA 2′-O-methylation dynamics impact cell fate decisions

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Ribosomal RNA 2′-O-methylation dynamics impact cell fate decisions. / Häfner, Sophia J.; Jansson, Martin D.; Altinel, Kübra; Andersen, Kasper L.; Abay-Nørgaard, Zehra; Ménard, Patrice; Fontenas, Martin; Sørensen, Daniel M.; Gay, David M.; Arendrup, Frederic S.; Tehler, Disa; Krogh, Nicolai; Nielsen, Henrik; Kraushar, Matthew L.; Kirkeby, Agnete; Lund, Anders H.

In: Developmental Cell, Vol. 58, No. 17, 2023, p. 1593-1609.e9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Häfner, SJ, Jansson, MD, Altinel, K, Andersen, KL, Abay-Nørgaard, Z, Ménard, P, Fontenas, M, Sørensen, DM, Gay, DM, Arendrup, FS, Tehler, D, Krogh, N, Nielsen, H, Kraushar, ML, Kirkeby, A & Lund, AH 2023, 'Ribosomal RNA 2′-O-methylation dynamics impact cell fate decisions', Developmental Cell, vol. 58, no. 17, pp. 1593-1609.e9. https://doi.org/10.1016/j.devcel.2023.06.007

APA

Häfner, S. J., Jansson, M. D., Altinel, K., Andersen, K. L., Abay-Nørgaard, Z., Ménard, P., Fontenas, M., Sørensen, D. M., Gay, D. M., Arendrup, F. S., Tehler, D., Krogh, N., Nielsen, H., Kraushar, M. L., Kirkeby, A., & Lund, A. H. (2023). Ribosomal RNA 2′-O-methylation dynamics impact cell fate decisions. Developmental Cell, 58(17), 1593-1609.e9. https://doi.org/10.1016/j.devcel.2023.06.007

Vancouver

Häfner SJ, Jansson MD, Altinel K, Andersen KL, Abay-Nørgaard Z, Ménard P et al. Ribosomal RNA 2′-O-methylation dynamics impact cell fate decisions. Developmental Cell. 2023;58(17):1593-1609.e9. https://doi.org/10.1016/j.devcel.2023.06.007

Author

Häfner, Sophia J. ; Jansson, Martin D. ; Altinel, Kübra ; Andersen, Kasper L. ; Abay-Nørgaard, Zehra ; Ménard, Patrice ; Fontenas, Martin ; Sørensen, Daniel M. ; Gay, David M. ; Arendrup, Frederic S. ; Tehler, Disa ; Krogh, Nicolai ; Nielsen, Henrik ; Kraushar, Matthew L. ; Kirkeby, Agnete ; Lund, Anders H. / Ribosomal RNA 2′-O-methylation dynamics impact cell fate decisions. In: Developmental Cell. 2023 ; Vol. 58, No. 17. pp. 1593-1609.e9.

Bibtex

@article{2611d43d1f444240aa92ea45e5b7ca96,
title = "Ribosomal RNA 2′-O-methylation dynamics impact cell fate decisions",
abstract = "Translational regulation impacts both pluripotency maintenance and cell differentiation. To what degree the ribosome exerts control over this process remains unanswered. Accumulating evidence has demonstrated heterogeneity in ribosome composition in various organisms. 2′-O-methylation (2′-O-me) of rRNA represents an important source of heterogeneity, where site-specific alteration of methylation levels can modulate translation. Here, we examine changes in rRNA 2′-O-me during mouse brain development and tri-lineage differentiation of human embryonic stem cells (hESCs). We find distinct alterations between brain regions, as well as clear dynamics during cortex development and germ layer differentiation. We identify a methylation site impacting neuronal differentiation. Modulation of its methylation levels affects ribosome association of the fragile X mental retardation protein (FMRP) and is accompanied by an altered translation of WNT pathway-related mRNAs. Together, these data identify ribosome heterogeneity through rRNA 2′-O-me during early development and differentiation and suggest a direct role for ribosomes in regulating translation during cell fate acquisition.",
keywords = "2′-O-methylation, brain development, cell fate, development, differentiation, human embryonic stem cells, neurogenesis, ribosome, RNA modifications, translation, translation programs",
author = "H{\"a}fner, {Sophia J.} and Jansson, {Martin D.} and K{\"u}bra Altinel and Andersen, {Kasper L.} and Zehra Abay-N{\o}rgaard and Patrice M{\'e}nard and Martin Fontenas and S{\o}rensen, {Daniel M.} and Gay, {David M.} and Arendrup, {Frederic S.} and Disa Tehler and Nicolai Krogh and Henrik Nielsen and Kraushar, {Matthew L.} and Agnete Kirkeby and Lund, {Anders H.}",
note = "Publisher Copyright: {\textcopyright} 2023 Elsevier Inc.",
year = "2023",
doi = "10.1016/j.devcel.2023.06.007",
language = "English",
volume = "58",
pages = "1593--1609.e9",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "17",

}

RIS

TY - JOUR

T1 - Ribosomal RNA 2′-O-methylation dynamics impact cell fate decisions

AU - Häfner, Sophia J.

AU - Jansson, Martin D.

AU - Altinel, Kübra

AU - Andersen, Kasper L.

AU - Abay-Nørgaard, Zehra

AU - Ménard, Patrice

AU - Fontenas, Martin

AU - Sørensen, Daniel M.

AU - Gay, David M.

AU - Arendrup, Frederic S.

AU - Tehler, Disa

AU - Krogh, Nicolai

AU - Nielsen, Henrik

AU - Kraushar, Matthew L.

AU - Kirkeby, Agnete

AU - Lund, Anders H.

N1 - Publisher Copyright: © 2023 Elsevier Inc.

PY - 2023

Y1 - 2023

N2 - Translational regulation impacts both pluripotency maintenance and cell differentiation. To what degree the ribosome exerts control over this process remains unanswered. Accumulating evidence has demonstrated heterogeneity in ribosome composition in various organisms. 2′-O-methylation (2′-O-me) of rRNA represents an important source of heterogeneity, where site-specific alteration of methylation levels can modulate translation. Here, we examine changes in rRNA 2′-O-me during mouse brain development and tri-lineage differentiation of human embryonic stem cells (hESCs). We find distinct alterations between brain regions, as well as clear dynamics during cortex development and germ layer differentiation. We identify a methylation site impacting neuronal differentiation. Modulation of its methylation levels affects ribosome association of the fragile X mental retardation protein (FMRP) and is accompanied by an altered translation of WNT pathway-related mRNAs. Together, these data identify ribosome heterogeneity through rRNA 2′-O-me during early development and differentiation and suggest a direct role for ribosomes in regulating translation during cell fate acquisition.

AB - Translational regulation impacts both pluripotency maintenance and cell differentiation. To what degree the ribosome exerts control over this process remains unanswered. Accumulating evidence has demonstrated heterogeneity in ribosome composition in various organisms. 2′-O-methylation (2′-O-me) of rRNA represents an important source of heterogeneity, where site-specific alteration of methylation levels can modulate translation. Here, we examine changes in rRNA 2′-O-me during mouse brain development and tri-lineage differentiation of human embryonic stem cells (hESCs). We find distinct alterations between brain regions, as well as clear dynamics during cortex development and germ layer differentiation. We identify a methylation site impacting neuronal differentiation. Modulation of its methylation levels affects ribosome association of the fragile X mental retardation protein (FMRP) and is accompanied by an altered translation of WNT pathway-related mRNAs. Together, these data identify ribosome heterogeneity through rRNA 2′-O-me during early development and differentiation and suggest a direct role for ribosomes in regulating translation during cell fate acquisition.

KW - 2′-O-methylation

KW - brain development

KW - cell fate

KW - development

KW - differentiation

KW - human embryonic stem cells

KW - neurogenesis

KW - ribosome

KW - RNA modifications

KW - translation

KW - translation programs

U2 - 10.1016/j.devcel.2023.06.007

DO - 10.1016/j.devcel.2023.06.007

M3 - Journal article

C2 - 37473757

AN - SCOPUS:85169830600

VL - 58

SP - 1593-1609.e9

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 17

ER -

ID: 367600954