Rac1 deletion causes thymic atrophy

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Rac1 deletion causes thymic atrophy. / Hunziker, Lukas; Benitah, Salvador Aznar; Aznar Benitah, Salvador; Braun, Kristin M; Jensen, Kim; McNulty, Katrina; Butler, Colin; Potton, Elspeth; Nye, Emma; Boyd, Richard; Laurent, Geoff; Glogauer, Michael; Wright, Nick A; Watt, Fiona M; Janes, Sam M.

In: PLOS ONE, Vol. 6, No. 4, 29.04.2011, p. e19292.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hunziker, L, Benitah, SA, Aznar Benitah, S, Braun, KM, Jensen, K, McNulty, K, Butler, C, Potton, E, Nye, E, Boyd, R, Laurent, G, Glogauer, M, Wright, NA, Watt, FM & Janes, SM 2011, 'Rac1 deletion causes thymic atrophy', PLOS ONE, vol. 6, no. 4, pp. e19292. https://doi.org/10.1371/journal.pone.0019292

APA

Hunziker, L., Benitah, S. A., Aznar Benitah, S., Braun, K. M., Jensen, K., McNulty, K., Butler, C., Potton, E., Nye, E., Boyd, R., Laurent, G., Glogauer, M., Wright, N. A., Watt, F. M., & Janes, S. M. (2011). Rac1 deletion causes thymic atrophy. PLOS ONE, 6(4), e19292. https://doi.org/10.1371/journal.pone.0019292

Vancouver

Hunziker L, Benitah SA, Aznar Benitah S, Braun KM, Jensen K, McNulty K et al. Rac1 deletion causes thymic atrophy. PLOS ONE. 2011 Apr 29;6(4):e19292. https://doi.org/10.1371/journal.pone.0019292

Author

Hunziker, Lukas ; Benitah, Salvador Aznar ; Aznar Benitah, Salvador ; Braun, Kristin M ; Jensen, Kim ; McNulty, Katrina ; Butler, Colin ; Potton, Elspeth ; Nye, Emma ; Boyd, Richard ; Laurent, Geoff ; Glogauer, Michael ; Wright, Nick A ; Watt, Fiona M ; Janes, Sam M. / Rac1 deletion causes thymic atrophy. In: PLOS ONE. 2011 ; Vol. 6, No. 4. pp. e19292.

Bibtex

@article{13f1b32f102b4ffc855bda1fc4587153,
title = "Rac1 deletion causes thymic atrophy",
abstract = "The thymic stroma supports T lymphocyte development and consists of an epithelium maintained by thymic epithelial progenitors. The molecular pathways that govern epithelial homeostasis are poorly understood. Here we demonstrate that deletion of Rac1 in Keratin 5/Keratin 14 expressing embryonic and adult thymic epithelial cells leads to loss of the thymic epithelial compartment. Rac1 deletion led to an increase in c-Myc expression and a generalized increase in apoptosis associated with a decrease in thymic epithelial proliferation. Our results suggest Rac1 maintains the epithelial population, and equilibrium between Rac1 and c-Myc may control proliferation, apoptosis and maturation of the thymic epithelial compartment. Understanding thymic epithelial maintenance is a step toward the dual goals of in vitro thymic epithelial cell culture and T cell differentiation, and the clinical repair of thymic damage from graft-versus-host-disease, chemotherapy or irradiation.",
keywords = "Animals, Atrophy, Cell Proliferation, Crosses, Genetic, Flow Cytometry, Gene Deletion, Gene Expression Regulation, Green Fluorescent Proteins/metabolism, Heterozygote, Homeostasis, Kidney/embryology, Mice, Microscopy, Fluorescence/methods, Neuropeptides/genetics, Promoter Regions, Genetic, Thymus Gland/embryology, Time Factors, rac GTP-Binding Proteins/genetics, rac1 GTP-Binding Protein",
author = "Lukas Hunziker and Benitah, {Salvador Aznar} and {Aznar Benitah}, Salvador and Braun, {Kristin M} and Kim Jensen and Katrina McNulty and Colin Butler and Elspeth Potton and Emma Nye and Richard Boyd and Geoff Laurent and Michael Glogauer and Wright, {Nick A} and Watt, {Fiona M} and Janes, {Sam M}",
year = "2011",
month = apr,
day = "29",
doi = "10.1371/journal.pone.0019292",
language = "English",
volume = "6",
pages = "e19292",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

RIS

TY - JOUR

T1 - Rac1 deletion causes thymic atrophy

AU - Hunziker, Lukas

AU - Benitah, Salvador Aznar

AU - Aznar Benitah, Salvador

AU - Braun, Kristin M

AU - Jensen, Kim

AU - McNulty, Katrina

AU - Butler, Colin

AU - Potton, Elspeth

AU - Nye, Emma

AU - Boyd, Richard

AU - Laurent, Geoff

AU - Glogauer, Michael

AU - Wright, Nick A

AU - Watt, Fiona M

AU - Janes, Sam M

PY - 2011/4/29

Y1 - 2011/4/29

N2 - The thymic stroma supports T lymphocyte development and consists of an epithelium maintained by thymic epithelial progenitors. The molecular pathways that govern epithelial homeostasis are poorly understood. Here we demonstrate that deletion of Rac1 in Keratin 5/Keratin 14 expressing embryonic and adult thymic epithelial cells leads to loss of the thymic epithelial compartment. Rac1 deletion led to an increase in c-Myc expression and a generalized increase in apoptosis associated with a decrease in thymic epithelial proliferation. Our results suggest Rac1 maintains the epithelial population, and equilibrium between Rac1 and c-Myc may control proliferation, apoptosis and maturation of the thymic epithelial compartment. Understanding thymic epithelial maintenance is a step toward the dual goals of in vitro thymic epithelial cell culture and T cell differentiation, and the clinical repair of thymic damage from graft-versus-host-disease, chemotherapy or irradiation.

AB - The thymic stroma supports T lymphocyte development and consists of an epithelium maintained by thymic epithelial progenitors. The molecular pathways that govern epithelial homeostasis are poorly understood. Here we demonstrate that deletion of Rac1 in Keratin 5/Keratin 14 expressing embryonic and adult thymic epithelial cells leads to loss of the thymic epithelial compartment. Rac1 deletion led to an increase in c-Myc expression and a generalized increase in apoptosis associated with a decrease in thymic epithelial proliferation. Our results suggest Rac1 maintains the epithelial population, and equilibrium between Rac1 and c-Myc may control proliferation, apoptosis and maturation of the thymic epithelial compartment. Understanding thymic epithelial maintenance is a step toward the dual goals of in vitro thymic epithelial cell culture and T cell differentiation, and the clinical repair of thymic damage from graft-versus-host-disease, chemotherapy or irradiation.

KW - Animals

KW - Atrophy

KW - Cell Proliferation

KW - Crosses, Genetic

KW - Flow Cytometry

KW - Gene Deletion

KW - Gene Expression Regulation

KW - Green Fluorescent Proteins/metabolism

KW - Heterozygote

KW - Homeostasis

KW - Kidney/embryology

KW - Mice

KW - Microscopy, Fluorescence/methods

KW - Neuropeptides/genetics

KW - Promoter Regions, Genetic

KW - Thymus Gland/embryology

KW - Time Factors

KW - rac GTP-Binding Proteins/genetics

KW - rac1 GTP-Binding Protein

U2 - 10.1371/journal.pone.0019292

DO - 10.1371/journal.pone.0019292

M3 - Journal article

C2 - 21559396

VL - 6

SP - e19292

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 4

ER -

ID: 200570885