Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function

Research output: Contribution to journalJournal articleResearchpeer-review

Multiciliated cells (MCCs) drive fluid flow in diverse tubular organs and are essential for the development and homeostasis of the vertebrate central nervous system, airway and reproductive tracts. These cells are characterized by dozens or hundreds of motile cilia that beat in a coordinated and polarized manner. In recent years, genomic studies have not only elucidated the transcriptional hierarchy for MCC specification but also identified myriad new proteins that govern MCC ciliogenesis, cilia beating and cilia polarization. Interestingly, this burst of genomic data has also highlighted that proteins with no obvious role in cilia do, in fact, have important ciliary functions. Understanding the function of proteins with little prior history of study presents a special challenge, especially when faced with large numbers of such proteins. Here, we define the subcellular localization in MCCs of ∼200 proteins not previously implicated in cilia biology. Functional analyses arising from the screen provide novel links between actin cytoskeleton and MCC ciliogenesis.

Original languageEnglish
Article number206565
JournalJournal of Cell Science
Volume131
Issue number3
Pages (from-to)1-10
Number of pages10
ISSN0021-9533
DOIs
Publication statusPublished - 29 Jan 2018

ID: 202029716