Organoids are not organs: Sources of variation and misinformation in organoid biology

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Organoids are not organs : Sources of variation and misinformation in organoid biology. / Jensen, Kim Bak; Little, Melissa Helen.

In: Stem Cell Reports, Vol. 18, No. 6, 2023, p. 1255-1270.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Jensen, KB & Little, MH 2023, 'Organoids are not organs: Sources of variation and misinformation in organoid biology', Stem Cell Reports, vol. 18, no. 6, pp. 1255-1270. https://doi.org/10.1016/j.stemcr.2023.05.009

APA

Jensen, K. B., & Little, M. H. (2023). Organoids are not organs: Sources of variation and misinformation in organoid biology. Stem Cell Reports, 18(6), 1255-1270. https://doi.org/10.1016/j.stemcr.2023.05.009

Vancouver

Jensen KB, Little MH. Organoids are not organs: Sources of variation and misinformation in organoid biology. Stem Cell Reports. 2023;18(6):1255-1270. https://doi.org/10.1016/j.stemcr.2023.05.009

Author

Jensen, Kim Bak ; Little, Melissa Helen. / Organoids are not organs : Sources of variation and misinformation in organoid biology. In: Stem Cell Reports. 2023 ; Vol. 18, No. 6. pp. 1255-1270.

Bibtex

@article{d1d2cfac33834566ad636cf5ba54b13b,
title = "Organoids are not organs: Sources of variation and misinformation in organoid biology",
abstract = "In the past decade, the term organoid has moved from obscurity to common use to describe a 3D in vitro cellular model of a tissue that recapitulates structural and functional elements of the in vivo organ it models. The term organoid is now applied to structures formed as a result of two distinct processes: the capacity for adult epithelial stem cells to re-create a tissue niche in vitro and the ability to direct the differentiation of pluripotent stem cells to a 3D self-organizing multicellular model of organogenesis. While these two organoid fields rely upon different stem cell types and recapitulate different processes, both share common challenges around robustness, accuracy, and reproducibility. Critically, organoids are not organs. This commentary serves to discuss these challenges, how they impact genuine utility, and shine a light on the need to improve the standards applied to all organoid approaches.",
keywords = "Adult, Humans, Reproducibility of Results, Organoids, Pluripotent Stem Cells, Communication, Biology",
author = "Jensen, {Kim Bak} and Little, {Melissa Helen}",
note = "Copyright {\textcopyright} 2023 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2023",
doi = "10.1016/j.stemcr.2023.05.009",
language = "English",
volume = "18",
pages = "1255--1270",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "6",

}

RIS

TY - JOUR

T1 - Organoids are not organs

T2 - Sources of variation and misinformation in organoid biology

AU - Jensen, Kim Bak

AU - Little, Melissa Helen

N1 - Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2023

Y1 - 2023

N2 - In the past decade, the term organoid has moved from obscurity to common use to describe a 3D in vitro cellular model of a tissue that recapitulates structural and functional elements of the in vivo organ it models. The term organoid is now applied to structures formed as a result of two distinct processes: the capacity for adult epithelial stem cells to re-create a tissue niche in vitro and the ability to direct the differentiation of pluripotent stem cells to a 3D self-organizing multicellular model of organogenesis. While these two organoid fields rely upon different stem cell types and recapitulate different processes, both share common challenges around robustness, accuracy, and reproducibility. Critically, organoids are not organs. This commentary serves to discuss these challenges, how they impact genuine utility, and shine a light on the need to improve the standards applied to all organoid approaches.

AB - In the past decade, the term organoid has moved from obscurity to common use to describe a 3D in vitro cellular model of a tissue that recapitulates structural and functional elements of the in vivo organ it models. The term organoid is now applied to structures formed as a result of two distinct processes: the capacity for adult epithelial stem cells to re-create a tissue niche in vitro and the ability to direct the differentiation of pluripotent stem cells to a 3D self-organizing multicellular model of organogenesis. While these two organoid fields rely upon different stem cell types and recapitulate different processes, both share common challenges around robustness, accuracy, and reproducibility. Critically, organoids are not organs. This commentary serves to discuss these challenges, how they impact genuine utility, and shine a light on the need to improve the standards applied to all organoid approaches.

KW - Adult

KW - Humans

KW - Reproducibility of Results

KW - Organoids

KW - Pluripotent Stem Cells

KW - Communication

KW - Biology

U2 - 10.1016/j.stemcr.2023.05.009

DO - 10.1016/j.stemcr.2023.05.009

M3 - Review

C2 - 37315519

VL - 18

SP - 1255

EP - 1270

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 6

ER -

ID: 356970812