Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling
Research output: Contribution to journal › Journal article › Research › peer-review
Similar to the brain, the eye is considered an immune-privileged organ where tissue-resident macrophages provide the major immune cell constituents. However, little is known about spatially restricted macrophage subsets within different eye compartments with regard to their origin, function, and fate during health and disease. Here, we combined single-cell analysis, fate mapping, parabiosis, and computational modeling to comprehensively examine myeloid subsets in distinct parts of the eye during homeostasis. This approach allowed us to identify myeloid subsets displaying diverse transcriptional states. During choroidal neovascularization, a typical hallmark of neovascular age-related macular degeneration (AMD), we recognized disease-specific macrophage subpopulations with distinct molecular signatures. Our results highlight the heterogeneity of myeloid subsets and their dynamics in the eye that provide new insights into the innate immune system in this organ which may offer new therapeutic targets for ophthalmological diseases.
Original language | English |
---|---|
Article number | e105123 |
Journal | EMBO Journal |
Volume | 40 |
Issue number | 6 |
ISSN | 0261-4189 |
DOIs | |
Publication status | Published - 2021 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:
© 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license
- cornea, macrophages, microglia, retina, single-cell RNA-seq
Research areas
ID: 331778573