Lrig1 expression defines a distinct multipotent stem cell population in mammalian epidermis
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Lrig1 expression defines a distinct multipotent stem cell population in mammalian epidermis. / Jensen, Kim B; Collins, Charlotte A; Nascimento, Elisabete; Tan, David W; Frye, Michaela; Itami, Satoshi; Watt, Fiona M.
In: Cell Stem Cell, Vol. 4, No. 5, 08.05.2009, p. 427-39.Research output: Contribution to journal › Journal article › Research
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TY - JOUR
T1 - Lrig1 expression defines a distinct multipotent stem cell population in mammalian epidermis
AU - Jensen, Kim B
AU - Collins, Charlotte A
AU - Nascimento, Elisabete
AU - Tan, David W
AU - Frye, Michaela
AU - Itami, Satoshi
AU - Watt, Fiona M
PY - 2009/5/8
Y1 - 2009/5/8
N2 - Lrig1 is a marker of human interfollicular epidermal stem cells and helps maintain stem cell quiescence. We show that, in mouse epidermis, Lrig1 defines the hair follicle junctional zone adjacent to the sebaceous glands and infundibulum. Lrig1 is a Myc target gene; loss of Lrig1 increases the proliferative capacity of stem cells in culture and results in epidermal hyperproliferation in vivo. Lrig1-expressing cells can give rise to all of the adult epidermal lineages in skin reconstitution assays. However, during homeostasis and on retinoic acid stimulation, they are bipotent, contributing to the sebaceous gland and interfollicular epidermis. beta-catenin activation increases the size of the junctional zone compartment, and loss of Lrig1 causes a selective increase in beta-catenin-induced ectopic hair follicle formation in the interfollicular epidermis. Our results suggest that Lrig1-positive cells constitute a previously unidentified reservoir of adult mouse interfollicular epidermal stem cells.
AB - Lrig1 is a marker of human interfollicular epidermal stem cells and helps maintain stem cell quiescence. We show that, in mouse epidermis, Lrig1 defines the hair follicle junctional zone adjacent to the sebaceous glands and infundibulum. Lrig1 is a Myc target gene; loss of Lrig1 increases the proliferative capacity of stem cells in culture and results in epidermal hyperproliferation in vivo. Lrig1-expressing cells can give rise to all of the adult epidermal lineages in skin reconstitution assays. However, during homeostasis and on retinoic acid stimulation, they are bipotent, contributing to the sebaceous gland and interfollicular epidermis. beta-catenin activation increases the size of the junctional zone compartment, and loss of Lrig1 causes a selective increase in beta-catenin-induced ectopic hair follicle formation in the interfollicular epidermis. Our results suggest that Lrig1-positive cells constitute a previously unidentified reservoir of adult mouse interfollicular epidermal stem cells.
KW - Animals
KW - Cell Differentiation
KW - Cell Lineage
KW - Cell Proliferation
KW - Epidermis
KW - Hair Follicle
KW - Mammals
KW - Membrane Glycoproteins
KW - Mice
KW - Mice, Transgenic
KW - Multipotent Stem Cells
KW - Nerve Tissue Proteins
KW - Proto-Oncogene Proteins c-myc
KW - beta Catenin
U2 - 10.1016/j.stem.2009.04.014
DO - 10.1016/j.stem.2009.04.014
M3 - Journal article
C2 - 19427292
VL - 4
SP - 427
EP - 439
JO - Cell Stem Cell
JF - Cell Stem Cell
SN - 1934-5909
IS - 5
ER -
ID: 94414716