Generation of multipotent foregut stem cells from human pluripotent stem cells
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Generation of multipotent foregut stem cells from human pluripotent stem cells. / Hannan, Nicholas R F; Fordham, Robert P; Syed, Yasir A; Moignard, Victoria; Berry, Andrew; Bautista, Ruben; Hanley, Neil A; Jensen, Kim B; Vallier, Ludovic.
In: Stem Cell Reviews, Vol. 1, No. 4, 2013, p. 293-306.Research output: Contribution to journal › Journal article › Research
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TY - JOUR
T1 - Generation of multipotent foregut stem cells from human pluripotent stem cells
AU - Hannan, Nicholas R F
AU - Fordham, Robert P
AU - Syed, Yasir A
AU - Moignard, Victoria
AU - Berry, Andrew
AU - Bautista, Ruben
AU - Hanley, Neil A
AU - Jensen, Kim B
AU - Vallier, Ludovic
PY - 2013
Y1 - 2013
N2 - Human pluripotent stem cells (hPSCs) could provide an infinite source of clinically relevant cells with potential applications in regenerative medicine. However, hPSC lines vary in their capacity to generate specialized cells, and the development of universal protocols for the production of tissue-specific cells remains a major challenge. Here, we have addressed this limitation for the endodermal lineage by developing a defined culture system to expand and differentiate human foregut stem cells (hFSCs) derived from hPSCs. hFSCs can self-renew while maintaining their capacity to differentiate into pancreatic and hepatic cells. Furthermore, near-homogenous populations of hFSCs can be obtained from hPSC lines which are normally refractory to endodermal differentiation. Therefore, hFSCs provide a unique approach to bypass variability between pluripotent lines in order to obtain a sustainable source of multipotent endoderm stem cells for basic studies and to produce a diversity of endodermal derivatives with a clinical value.
AB - Human pluripotent stem cells (hPSCs) could provide an infinite source of clinically relevant cells with potential applications in regenerative medicine. However, hPSC lines vary in their capacity to generate specialized cells, and the development of universal protocols for the production of tissue-specific cells remains a major challenge. Here, we have addressed this limitation for the endodermal lineage by developing a defined culture system to expand and differentiate human foregut stem cells (hFSCs) derived from hPSCs. hFSCs can self-renew while maintaining their capacity to differentiate into pancreatic and hepatic cells. Furthermore, near-homogenous populations of hFSCs can be obtained from hPSC lines which are normally refractory to endodermal differentiation. Therefore, hFSCs provide a unique approach to bypass variability between pluripotent lines in order to obtain a sustainable source of multipotent endoderm stem cells for basic studies and to produce a diversity of endodermal derivatives with a clinical value.
U2 - 10.1016/j.stemcr.2013.09.003
DO - 10.1016/j.stemcr.2013.09.003
M3 - Journal article
C2 - 24319665
VL - 1
SP - 293
EP - 306
JO - Stem Cell Reviews
JF - Stem Cell Reviews
SN - 1550-8943
IS - 4
ER -
ID: 94418485