Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

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Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol. / Kirkeby, Agnete; Nelander, Jenny; Parmar, Malin.

In: Frontiers in Cellular Neuroscience, No. JANUARY 2013, 03.01.2013.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kirkeby, A, Nelander, J & Parmar, M 2013, 'Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol', Frontiers in Cellular Neuroscience, no. JANUARY 2013. https://doi.org/10.3389/fncel.2012.00064

APA

Kirkeby, A., Nelander, J., & Parmar, M. (2013). Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol. Frontiers in Cellular Neuroscience, (JANUARY 2013). https://doi.org/10.3389/fncel.2012.00064

Vancouver

Kirkeby A, Nelander J, Parmar M. Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol. Frontiers in Cellular Neuroscience. 2013 Jan 3;(JANUARY 2013). https://doi.org/10.3389/fncel.2012.00064

Author

Kirkeby, Agnete ; Nelander, Jenny ; Parmar, Malin. / Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol. In: Frontiers in Cellular Neuroscience. 2013 ; No. JANUARY 2013.

Bibtex

@article{422d232ee3d64c83870ad3851e4c58c2,
title = "Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol",
abstract = "Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.",
keywords = "Brain development, Differentiation, Gsk3, Human embryonic stem cells, Neuronal subtypes, Pluripotent stem cells, Protocol, Regionalization",
author = "Agnete Kirkeby and Jenny Nelander and Malin Parmar",
year = "2013",
month = jan,
day = "3",
doi = "10.3389/fncel.2012.00064",
language = "English",
journal = "Frontiers in Cellular Neuroscience",
issn = "1662-5102",
publisher = "Frontiers Media S.A.",
number = "JANUARY 2013",

}

RIS

TY - JOUR

T1 - Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

AU - Kirkeby, Agnete

AU - Nelander, Jenny

AU - Parmar, Malin

PY - 2013/1/3

Y1 - 2013/1/3

N2 - Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.

AB - Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.

KW - Brain development

KW - Differentiation

KW - Gsk3

KW - Human embryonic stem cells

KW - Neuronal subtypes

KW - Pluripotent stem cells

KW - Protocol

KW - Regionalization

UR - http://www.scopus.com/inward/record.url?scp=84872186092&partnerID=8YFLogxK

U2 - 10.3389/fncel.2012.00064

DO - 10.3389/fncel.2012.00064

M3 - Journal article

AN - SCOPUS:84872186092

JO - Frontiers in Cellular Neuroscience

JF - Frontiers in Cellular Neuroscience

SN - 1662-5102

IS - JANUARY 2013

ER -

ID: 228506201