Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo

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Standard

Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo. / Nyeng, Pia; Dela Cruz, Gelo Victoriano; Semb, Tor Henrik.

In: STAR Protocols, Vol. 2, No. 3, 100636, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nyeng, P, Dela Cruz, GV & Semb, TH 2021, 'Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo', STAR Protocols, vol. 2, no. 3, 100636. https://doi.org/10.1016/j.xpro.2021.100636

APA

Nyeng, P., Dela Cruz, G. V., & Semb, T. H. (2021). Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo. STAR Protocols, 2(3), [100636]. https://doi.org/10.1016/j.xpro.2021.100636

Vancouver

Nyeng P, Dela Cruz GV, Semb TH. Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo. STAR Protocols. 2021;2(3). 100636. https://doi.org/10.1016/j.xpro.2021.100636

Author

Nyeng, Pia ; Dela Cruz, Gelo Victoriano ; Semb, Tor Henrik. / Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo. In: STAR Protocols. 2021 ; Vol. 2, No. 3.

Bibtex

@article{670f6be130aa4ceeb4b7991d5beaeddd,
title = "Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo",
abstract = "We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).",
author = "Pia Nyeng and {Dela Cruz}, {Gelo Victoriano} and Semb, {Tor Henrik}",
note = "{\textcopyright} 2021 The Author(s).",
year = "2021",
doi = "10.1016/j.xpro.2021.100636",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo

AU - Nyeng, Pia

AU - Dela Cruz, Gelo Victoriano

AU - Semb, Tor Henrik

N1 - © 2021 The Author(s).

PY - 2021

Y1 - 2021

N2 - We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).

AB - We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).

U2 - 10.1016/j.xpro.2021.100636

DO - 10.1016/j.xpro.2021.100636

M3 - Journal article

C2 - 34258596

VL - 2

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 3

M1 - 100636

ER -

ID: 284836691