Epsilon haemoglobin specific antibodies with applications in noninvasive prenatal diagnosis
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Epsilon haemoglobin specific antibodies with applications in noninvasive prenatal diagnosis. / Sørensen, Morten Draeby; Gonzalez Dosal, Regina; Jensen, Kim Bak; Christensen, Britta; Kølvraa, Steen; Jensen, Uffe Birk.
In: Journal of Biomedicine and Biotechnology, Vol. 2009, 2009, p. 659219.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Epsilon haemoglobin specific antibodies with applications in noninvasive prenatal diagnosis
AU - Sørensen, Morten Draeby
AU - Gonzalez Dosal, Regina
AU - Jensen, Kim Bak
AU - Christensen, Britta
AU - Kølvraa, Steen
AU - Jensen, Uffe Birk
PY - 2009
Y1 - 2009
N2 - Invasive procedures for prenatal diagnosis are associated with increased risk of abortion; thus, development of noninvasive procedures would be beneficial. Based on the observation that embryonic nucleated red blood cell (NRBC) crosses the placenta and enters the circulation of pregnant women, the ability to identify such cell would allow development of such procedures. Identification of NRBCs in blood samples would be possible provided that specific antibodies are available. Here we have isolated recombinant antibodies using phage display. From the panel of antibody fragments specifically recognising epsilon-Hb, one was chosen for further characterization, DAb1. DAb1 binds to epsilon-Hb both in Western blots and immunocytochemistry. Several epsilon-Hb positive cells were detected in a blood sample taken as postchorionic villus sampling (CVS). To evaluate the sensitivity of the method, K562 cells (which express epsilon-Hb) were spiked in a blood sample followed by staining in solution and FACS analysis.
AB - Invasive procedures for prenatal diagnosis are associated with increased risk of abortion; thus, development of noninvasive procedures would be beneficial. Based on the observation that embryonic nucleated red blood cell (NRBC) crosses the placenta and enters the circulation of pregnant women, the ability to identify such cell would allow development of such procedures. Identification of NRBCs in blood samples would be possible provided that specific antibodies are available. Here we have isolated recombinant antibodies using phage display. From the panel of antibody fragments specifically recognising epsilon-Hb, one was chosen for further characterization, DAb1. DAb1 binds to epsilon-Hb both in Western blots and immunocytochemistry. Several epsilon-Hb positive cells were detected in a blood sample taken as postchorionic villus sampling (CVS). To evaluate the sensitivity of the method, K562 cells (which express epsilon-Hb) were spiked in a blood sample followed by staining in solution and FACS analysis.
KW - Antibodies/chemistry
KW - Blotting, Western
KW - Erythroblasts/cytology
KW - Female
KW - Fetal Blood/cytology
KW - Flow Cytometry
KW - Humans
KW - In Situ Hybridization, Fluorescence
KW - K562 Cells
KW - Peptide Library
KW - Pregnancy
KW - Prenatal Diagnosis/methods
KW - Recombinant Proteins/chemistry
KW - Sensitivity and Specificity
KW - epsilon-Globins/analysis
U2 - 10.1155/2009/659219
DO - 10.1155/2009/659219
M3 - Journal article
C2 - 19636421
VL - 2009
SP - 659219
JO - BioMed Research International
JF - BioMed Research International
SN - 2314-6133
ER -
ID: 200571464