Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation

Research output: Contribution to journal › Journal article › Research

Standard

Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation. / Baker, Christopher M; Verstuyf, Annemieke; Jensen, Kim B; Watt, Fiona M.

In: Developmental Biology, Vol. 343, No. 1-2, 01.07.2010, p. 40-50.

Research output: Contribution to journal › Journal article › Research

Harvard

Baker, CM, Verstuyf, A, Jensen, KB & Watt, FM 2010, 'Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation', Developmental Biology, vol. 343, no. 1-2, pp. 40-50. https://doi.org/10.1016/j.ydbio.2010.04.005

APA

Baker, C. M., Verstuyf, A., Jensen, K. B., & Watt, F. M. (2010). Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation. Developmental Biology, 343(1-2), 40-50. https://doi.org/10.1016/j.ydbio.2010.04.005

Vancouver

Baker CM, Verstuyf A, Jensen KB, Watt FM. Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation. Developmental Biology. 2010 Jul 1;343(1-2):40-50. https://doi.org/10.1016/j.ydbio.2010.04.005

Author

Baker, Christopher M ; Verstuyf, Annemieke ; Jensen, Kim B ; Watt, Fiona M. / Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation. In: Developmental Biology. 2010 ; Vol. 343, No. 1-2. pp. 40-50.

Bibtex

@article{3ec28c959c0d4628a8573f7d7ac17648,
title = "Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation",
abstract = "Wnt signalling is required for hair follicle development and for the growth phase (anagen) of postnatal follicles. When the pathway is activated at high levels in adult mouse epidermis, ectopic follicles form from existing follicles, interfollicular epidermis (IFE) and sebaceous glands, revealing a remarkable ability of the tissue to be reprogrammed. To compare the competence of different epidermal cell populations to form ectopic follicles, we expressed a 4-hydroxy-tamoxifen (4OHT) inducible, stabilised beta-catenin transgene (DeltaNbeta-cateninER) under the control of two different promoters. We targeted the reservoir of stem cells in the hair follicle bulge via the keratin 15 (K15) promoter and targeted the sebaceous glands and base of the follicle (bulb) with a truncated K5 promoter (DeltaK5). No ectopic follicles formed in the IFE in either model, establishing the autonomy of the IFE stem cell compartment in undamaged epidermis. Activation of beta-catenin in the bulge stimulated proliferation and bulge expansion. Existing hair follicles entered anagen, but no ectopic follicles formed. DeltaK5DeltaNbeta-cateninER expressing hair follicles also entered anagen on 4OHT treatment. In addition, a subpopulation of cells at the base of the sebaceous gland readily formed ectopic follicles, resulting in complete and reversible conversion of sebaceous glands into hair follicles. Combined activation of beta-catenin and the vitamin D receptor enhanced differentiation of sebaceous gland-derived hair follicles and stimulated ectopic follicle formation in the hair follicle bulb, but not in the bulge. Our results suggest that the bulge and sebaceous gland are, respectively, non-permissive and permissive niches for Wnt induced hair follicle differentiation.",
keywords = "Animals, Cell Differentiation, Cell Lineage, Epidermis, Epithelial Cells, Female, Hair Follicle, Male, Mice, Mice, Transgenic, beta Catenin",
author = "Baker, {Christopher M} and Annemieke Verstuyf and Jensen, {Kim B} and Watt, {Fiona M}",
note = "Copyright 2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = jul,
day = "1",
doi = "10.1016/j.ydbio.2010.04.005",
language = "English",
volume = "343",
pages = "40--50",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press",
number = "1-2",

}

RIS

TY - JOUR

T1 - Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation

AU - Baker, Christopher M

AU - Verstuyf, Annemieke

AU - Jensen, Kim B

AU - Watt, Fiona M

N1 - Copyright 2010 Elsevier Inc. All rights reserved.

PY - 2010/7/1

Y1 - 2010/7/1

N2 - Wnt signalling is required for hair follicle development and for the growth phase (anagen) of postnatal follicles. When the pathway is activated at high levels in adult mouse epidermis, ectopic follicles form from existing follicles, interfollicular epidermis (IFE) and sebaceous glands, revealing a remarkable ability of the tissue to be reprogrammed. To compare the competence of different epidermal cell populations to form ectopic follicles, we expressed a 4-hydroxy-tamoxifen (4OHT) inducible, stabilised beta-catenin transgene (DeltaNbeta-cateninER) under the control of two different promoters. We targeted the reservoir of stem cells in the hair follicle bulge via the keratin 15 (K15) promoter and targeted the sebaceous glands and base of the follicle (bulb) with a truncated K5 promoter (DeltaK5). No ectopic follicles formed in the IFE in either model, establishing the autonomy of the IFE stem cell compartment in undamaged epidermis. Activation of beta-catenin in the bulge stimulated proliferation and bulge expansion. Existing hair follicles entered anagen, but no ectopic follicles formed. DeltaK5DeltaNbeta-cateninER expressing hair follicles also entered anagen on 4OHT treatment. In addition, a subpopulation of cells at the base of the sebaceous gland readily formed ectopic follicles, resulting in complete and reversible conversion of sebaceous glands into hair follicles. Combined activation of beta-catenin and the vitamin D receptor enhanced differentiation of sebaceous gland-derived hair follicles and stimulated ectopic follicle formation in the hair follicle bulb, but not in the bulge. Our results suggest that the bulge and sebaceous gland are, respectively, non-permissive and permissive niches for Wnt induced hair follicle differentiation.

AB - Wnt signalling is required for hair follicle development and for the growth phase (anagen) of postnatal follicles. When the pathway is activated at high levels in adult mouse epidermis, ectopic follicles form from existing follicles, interfollicular epidermis (IFE) and sebaceous glands, revealing a remarkable ability of the tissue to be reprogrammed. To compare the competence of different epidermal cell populations to form ectopic follicles, we expressed a 4-hydroxy-tamoxifen (4OHT) inducible, stabilised beta-catenin transgene (DeltaNbeta-cateninER) under the control of two different promoters. We targeted the reservoir of stem cells in the hair follicle bulge via the keratin 15 (K15) promoter and targeted the sebaceous glands and base of the follicle (bulb) with a truncated K5 promoter (DeltaK5). No ectopic follicles formed in the IFE in either model, establishing the autonomy of the IFE stem cell compartment in undamaged epidermis. Activation of beta-catenin in the bulge stimulated proliferation and bulge expansion. Existing hair follicles entered anagen, but no ectopic follicles formed. DeltaK5DeltaNbeta-cateninER expressing hair follicles also entered anagen on 4OHT treatment. In addition, a subpopulation of cells at the base of the sebaceous gland readily formed ectopic follicles, resulting in complete and reversible conversion of sebaceous glands into hair follicles. Combined activation of beta-catenin and the vitamin D receptor enhanced differentiation of sebaceous gland-derived hair follicles and stimulated ectopic follicle formation in the hair follicle bulb, but not in the bulge. Our results suggest that the bulge and sebaceous gland are, respectively, non-permissive and permissive niches for Wnt induced hair follicle differentiation.

KW - Animals

KW - Cell Differentiation

KW - Cell Lineage

KW - Epidermis

KW - Epithelial Cells

KW - Female

KW - Hair Follicle

KW - Male

KW - Mice

KW - Mice, Transgenic

KW - beta Catenin

U2 - 10.1016/j.ydbio.2010.04.005

DO - 10.1016/j.ydbio.2010.04.005

M3 - Journal article

C2 - 20398648

VL - 343

SP - 40

EP - 50

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1-2

ER -

ID: 94414337