Comprehensive analysis of microrna expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor
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Comprehensive analysis of microrna expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor. / Jönsson, Marie E.; Wahlestedt, Jenny Nelander; Åkerblom, Malin; Kirkeby, Agnete; Malmevik, Josephine; Brattaas, Per Ludvik; Jakobsson, Johan; Parmar, Malin.
In: Development (Cambridge), Vol. 142, No. 18, 23.09.2015, p. 3166-3177.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Comprehensive analysis of microrna expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor
AU - Jönsson, Marie E.
AU - Wahlestedt, Jenny Nelander
AU - Åkerblom, Malin
AU - Kirkeby, Agnete
AU - Malmevik, Josephine
AU - Brattaas, Per Ludvik
AU - Jakobsson, Johan
AU - Parmar, Malin
PY - 2015/9/23
Y1 - 2015/9/23
N2 - MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number of genes enriched for functions including transcription, actin cytoskeleton and ephrin receptor signalling. When overexpressed, miR-10 influences caudalization of human neural progenitor cells. Together, these data confirm a role for miRNAs in establishing different human neural progenitor populations. This dataset also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development.
AB - MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number of genes enriched for functions including transcription, actin cytoskeleton and ephrin receptor signalling. When overexpressed, miR-10 influences caudalization of human neural progenitor cells. Together, these data confirm a role for miRNAs in establishing different human neural progenitor populations. This dataset also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development.
KW - Cell specification
KW - MicroRNA
KW - Neural development
KW - Neural stem cells
UR - http://www.scopus.com/inward/record.url?scp=84942163494&partnerID=8YFLogxK
U2 - 10.1242/dev.122747
DO - 10.1242/dev.122747
M3 - Journal article
C2 - 26395143
AN - SCOPUS:84942163494
VL - 142
SP - 3166
EP - 3177
JO - Development
JF - Development
SN - 0950-1991
IS - 18
ER -
ID: 228505790