A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation. / Ostrop, Jenny; Zwiggelaar, Rosalie T.; Terndrup Pedersen, Marianne; Gerbe, François; Bösl, Korbinian; Lindholm, Håvard T.; Díez-Sánchez, Alberto; Parmar, Naveen; Radetzki, Silke; von Kries, Jens Peter; Jay, Philippe; Jensen, Kim B.; Arrowsmith, Cheryl; Oudhoff, Menno J.

In: Frontiers in Cell and Developmental Biology, Vol. 8, 618552, 2021, p. 1-20.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ostrop, J, Zwiggelaar, RT, Terndrup Pedersen, M, Gerbe, F, Bösl, K, Lindholm, HT, Díez-Sánchez, A, Parmar, N, Radetzki, S, von Kries, JP, Jay, P, Jensen, KB, Arrowsmith, C & Oudhoff, MJ 2021, 'A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation', Frontiers in Cell and Developmental Biology, vol. 8, 618552, pp. 1-20. https://doi.org/10.3389/fcell.2020.618552

APA

Ostrop, J., Zwiggelaar, R. T., Terndrup Pedersen, M., Gerbe, F., Bösl, K., Lindholm, H. T., Díez-Sánchez, A., Parmar, N., Radetzki, S., von Kries, J. P., Jay, P., Jensen, K. B., Arrowsmith, C., & Oudhoff, M. J. (2021). A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation. Frontiers in Cell and Developmental Biology, 8, 1-20. [618552]. https://doi.org/10.3389/fcell.2020.618552

Vancouver

Ostrop J, Zwiggelaar RT, Terndrup Pedersen M, Gerbe F, Bösl K, Lindholm HT et al. A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation. Frontiers in Cell and Developmental Biology. 2021;8:1-20. 618552. https://doi.org/10.3389/fcell.2020.618552

Author

Ostrop, Jenny ; Zwiggelaar, Rosalie T. ; Terndrup Pedersen, Marianne ; Gerbe, François ; Bösl, Korbinian ; Lindholm, Håvard T. ; Díez-Sánchez, Alberto ; Parmar, Naveen ; Radetzki, Silke ; von Kries, Jens Peter ; Jay, Philippe ; Jensen, Kim B. ; Arrowsmith, Cheryl ; Oudhoff, Menno J. / A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation. In: Frontiers in Cell and Developmental Biology. 2021 ; Vol. 8. pp. 1-20.

Bibtex

@article{b07701b6369142b08dc89fdac066855a,
title = "A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation",
abstract = "Intestinal organoids are an excellent model to study epithelial biology. Yet, the selection of analytical tools to accurately quantify heterogeneous organoid cultures remains limited. Here, we developed a semi-automated organoid screening method, which we applied to a library of highly specific chemical probes to identify epigenetic regulators of intestinal epithelial biology. The role of epigenetic modifiers in adult stem cell systems, such as the intestinal epithelium, is still undefined. Based on this resource dataset, we identified several targets that affected epithelial cell differentiation, including HDACs, EP300/CREBBP, LSD1, and type I PRMTs, which were verified by complementary methods. For example, we show that inhibiting type I PRMTs, which leads enhanced epithelial differentiation, blocks the growth of adenoma but not normal organoid cultures. Thus, epigenetic probes are powerful tools to study intestinal epithelial biology and may have therapeutic potential.",
keywords = "bioimage analysis, CREBBP, EP300, epigenetic modifiers, intestinal stem cell biology, organoids, PRMT1",
author = "Jenny Ostrop and Zwiggelaar, {Rosalie T.} and {Terndrup Pedersen}, Marianne and Fran{\c c}ois Gerbe and Korbinian B{\"o}sl and Lindholm, {H{\aa}vard T.} and Alberto D{\'i}ez-S{\'a}nchez and Naveen Parmar and Silke Radetzki and {von Kries}, {Jens Peter} and Philippe Jay and Jensen, {Kim B.} and Cheryl Arrowsmith and Oudhoff, {Menno J.}",
year = "2021",
doi = "10.3389/fcell.2020.618552",
language = "English",
volume = "8",
pages = "1--20",
journal = "Frontiers in Cell and Developmental Biology",
issn = "2296-634X",
publisher = "Frontiers Media",

}

RIS

TY - JOUR

T1 - A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation

AU - Ostrop, Jenny

AU - Zwiggelaar, Rosalie T.

AU - Terndrup Pedersen, Marianne

AU - Gerbe, François

AU - Bösl, Korbinian

AU - Lindholm, Håvard T.

AU - Díez-Sánchez, Alberto

AU - Parmar, Naveen

AU - Radetzki, Silke

AU - von Kries, Jens Peter

AU - Jay, Philippe

AU - Jensen, Kim B.

AU - Arrowsmith, Cheryl

AU - Oudhoff, Menno J.

PY - 2021

Y1 - 2021

N2 - Intestinal organoids are an excellent model to study epithelial biology. Yet, the selection of analytical tools to accurately quantify heterogeneous organoid cultures remains limited. Here, we developed a semi-automated organoid screening method, which we applied to a library of highly specific chemical probes to identify epigenetic regulators of intestinal epithelial biology. The role of epigenetic modifiers in adult stem cell systems, such as the intestinal epithelium, is still undefined. Based on this resource dataset, we identified several targets that affected epithelial cell differentiation, including HDACs, EP300/CREBBP, LSD1, and type I PRMTs, which were verified by complementary methods. For example, we show that inhibiting type I PRMTs, which leads enhanced epithelial differentiation, blocks the growth of adenoma but not normal organoid cultures. Thus, epigenetic probes are powerful tools to study intestinal epithelial biology and may have therapeutic potential.

AB - Intestinal organoids are an excellent model to study epithelial biology. Yet, the selection of analytical tools to accurately quantify heterogeneous organoid cultures remains limited. Here, we developed a semi-automated organoid screening method, which we applied to a library of highly specific chemical probes to identify epigenetic regulators of intestinal epithelial biology. The role of epigenetic modifiers in adult stem cell systems, such as the intestinal epithelium, is still undefined. Based on this resource dataset, we identified several targets that affected epithelial cell differentiation, including HDACs, EP300/CREBBP, LSD1, and type I PRMTs, which were verified by complementary methods. For example, we show that inhibiting type I PRMTs, which leads enhanced epithelial differentiation, blocks the growth of adenoma but not normal organoid cultures. Thus, epigenetic probes are powerful tools to study intestinal epithelial biology and may have therapeutic potential.

KW - bioimage analysis

KW - CREBBP

KW - EP300

KW - epigenetic modifiers

KW - intestinal stem cell biology

KW - organoids

KW - PRMT1

U2 - 10.3389/fcell.2020.618552

DO - 10.3389/fcell.2020.618552

M3 - Journal article

C2 - 33575256

AN - SCOPUS:85101007775

VL - 8

SP - 1

EP - 20

JO - Frontiers in Cell and Developmental Biology

JF - Frontiers in Cell and Developmental Biology

SN - 2296-634X

M1 - 618552

ER -

ID: 257653729