2024.01.19
Serup Group - Developmental Biology of the Pancreas
The Serup lab investigates how cell-cell signalling interfaces with lineage-specific transcription factors to regulate cell fate decisions. The major focus of the lab is to understand how Notch signalling controls progenitor behaviour and differentiation in the developing pancreas.
The group is particularly interested in understanding how Notch signalling regulates several distinct processes during pancreatic development. They unravel how gene regulatory network architecture ensures robust cell fate regulation and how ultradian oscillations in gene activity are linked to cell proliferation and fate choice. They explore the generality of their findings in other organ systems.
Most of their studies are done with mice and human pluripotent stem cells, using a wide variety of techniques including gene targeting, transgenic mice, tissue explants, NGS-based assays, immunohistochemistry, confocal microscopy, and time-lapse imaging.
The aim of all these experiments is to understand the genetic and cellular interactions that direct pancreatic development in vivo and in vitro during differentiation of human pluripotent stem cells.
- To determine whether and how different Notch ligands regulate different processes in pancreas development and whether redundancy exist between different ligands.
- To elucidate the gene regulatory network architecture downstream of Notch signalling in order to understand how it ensures robust regulation of cell fate in pancreatic development.
- To determine how tissue geometry and Notch signalling interact to ensure proximodistal patterning of the pancreas.
- To develop improved protocols for directed differentiation of human pluripotent stem cells towards insulin-producing β-cells for use in cell-based diabetes therapy.
- To develop novel computational tools that will facilitate understanding of the above-mentioned processes.
- Xu et al. 2023. Jag1-Notch cis-interaction determines cell fate segregation in pancreatic development. Nat. Commun. 14: 348. doi: 10.1038/s41467-023-35963-w.
- Seymour et al. 2020. Jag1 Modulates an Oscillatory Dll1-Notch-Hes1 Signaling Module to Coordinate Growth and Fate of Pancreatic Progenitors. Dev. Cell. 52: 731-747. doi: 10.1016/j.devcel.2020.01.015.
- Jørgensen et al. 2018. Neurog3-dependent pancreas dysgenesis causes ectopic pancreas in Hes1 mutant mice. Development 145: dev163568. doi: 10.1242/dev.163568.
- Horn et al. 2012. Mind bomb 1 is required for pancreatic β-cell formation. Proc. Natl. Acad. Sci. U S A. 109: 7356-61. doi: 10.1073/pnas.1203605109.
- Ahnfelt-Rønne et al. 2012. Ptf1a-mediated control of Dll1 reveals an alternative to the lateral inhibition mechanism. Development 139: 33-45. doi: 10.1242/dev.071761.
- Ryoichiro Kageyama, RIKEN Center for Brain Science, Hirosawa Wako City, Japan
- Jorge Ferrer, Center for Genomic Regulation, Barcelona, Spain.
- Gerard Gradwohl, IGBMC, Strasbourg, France.
- Meritxell Huch, MPI-CBG, Dresden, Germany.
- Danwei Huangfu, MSKCC, New York, USA.
- Josh Brickman, NNF Center for Stem Cell Medicine, Copenhagen, Denmark.
- Kim B. Jensen, NNF Center for Stem Cell Medicine, Copenhagen, Denmark.
- Agnete Kirkeby, NNF Center for Stem Cell Medicine, Copenhagen, Denmark.
- Novo Nordisk Foundation Nordic Endocrinology 2020 - 2024 for the project: ''Decoding Notch signaling - The importance of cycling at the right pace''
- Novo Nordisk Foundation Center for Stem Cell Medicine (NNF21CC0073729)
Staff List - Serup Group
| Name | Title | Job responsibilities | Phone | |
|---|---|---|---|---|
| Search in Name | Search in Title | Search in Job responsibilities | Search in Phone | |
| Jørgensen, Mette Christine | Staff Scientist | Staff Scientist | ||
| Mjøseng, Heidi Katharina | Academic Research Staff | +4535333829 | ||
| Nissen, Silas Boye | International Researcher | Postdoc | ||
| Pajuelo Reyes, Cecilia Isabel | PhD Fellow | +4535325826 | ||
| Quaranta, Roberto | Assistant Professor | |||
| Raineri, Silvia | Postdoc | |||
| Seymour, Philip Allan | Assistant Professor | |||
| Vidal I Pitarch, Berta | Academic Research Staff | |||
| Xu, Xiaochan | Assistant Professor | Postdoc | +4535326306 |